![]() Not for resale without express authorization. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.įor Research Use Only. Associations between GAPDH, actin and tubulin have also be reported. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH Antibody (6C5) is an IgG1 mouse monoclonal GAPDH antibody that detects GAPDH of mouse, rat and human origin by WB, IP and IF. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. GAPDH and COXIV or cytochrome C for cytosolic and mitochondrial fractions respectively ( Dimauro et al. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. It is therefore crucial to assess the relative purity for fractions isolation by performing a WB for proteins known to be associated with the desired location (e.g. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Note : For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of What are the known protein-protein interactions of GAPDH? PVDF Membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody (Catalog MAB5718) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog HAF007). Useful as a control for Western blots and RT-PCR. Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus Is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and actingĪs a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in ![]() GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, evenĬritical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |